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mouse fibroblast cell line l929  (DSMZ)


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    Structured Review

    DSMZ mouse fibroblast cell line l929
    Mouse Fibroblast Cell Line L929, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/l+929+mouse+fibroblasts/L-929/pm41893202-115-8-13
    Average 95 stars, based on 253 article reviews
    mouse fibroblast cell line l929 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Iodine-Promoted Oxidative Conversion ofo-Vinyl Diaryl Ketones intoo-Acetyl Diaryl Ketones, Synthesis of 1-Methyl-4-arylphthalazines as Analogues of Podophyllotoxin
    Article Snippet: 1,2-Diacylbenzenes 1 (see Figure 1) have been valuable precursors for variety of heterocyclic scaffolds such as isoindoles, isoquinolines, N-arylphthalimidines, and phthalazines, which are known for their significance in the areas of biology and pharmaceuticals.. [1] Although a general method to synthesize this class of compounds is lacking, Kotali’s protocol, which involves Pb(OAc)4 promoted rearrangements of monoacylhydrazones of 2-hydroxyarylaldehydes and -ketones, constitutes a good route for the synthesis of 1.. [2] The limited availability of prefunctionalized starting materials and the toxicity of lead reagents restrict the employment of this method.

    Article Title: HLE-inhibitory alkaloids with a polyketide skeleton from the marine-derived fungus Coniothyrium cereale.
    Article Snippet: .. An MTT assay was used to measure the influence of compounds on the propagation and viability of L-929 mouse fibroblasts (DSMZ ACC2) in 96-well plates. .. Cells are able to reduce MTT [3-(4,5-dimethylthiazol-2-yl)2,5diphenyltetrazolium bromide] (Sigma) to a violet formazan product.

    Article Title: The Role of Formamidine Groups in Dextran Based Nonviral Vectors for Gene Delivery on Their Physicochemical and Biological Characteristics.
    Article Snippet: .. In Vitro Cytotoxicity Test: In vitro cytotoxicity of the polymers was tested by the MTT assay[56,48] using L-929 mouse fibroblasts (ACC 2, DSMZ) cultured in RPMI (Roswell Park Memorial Institute) 1 640 culture medium (Gibco, Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal bovine serum at 37 °C, 5% CO2 and 95% relative humidity. .. Briefly, after 24 h preincubation cells (8500 per well, Cellstar 96-well plates, Greiner Bio-One GmbH, Frickenhausen, Germany) were incubated with serial dilutions of the polymers (3.91 μg mL−1 to 500 μg mL−1) for 4 h. After removal of the supernatant and replacement by 200 μL serum containing RPMI 1640 medium cells were incubated for further 20 h. Following the incubation with MTT solution (500 μg mL−1), the absorbance of the samples was measured in a microplate reader (Spark 10M multimode microplate reader, Tecan Group AG) at 570 nm.

    Article Title: Antimicrobial phenalenone derivatives from the marine-derived fungus Coniothyrium cereale.
    Article Snippet: The marine-derived fungus Coniothyrium cereale was isolated from the green alga Enteromorpha sp. and found to produce the new phenalenone derivatives 1–7 as well as the known compounds lactone 8, (-) sclerodin (9), lamellicolic anhydride (10), (-) scleroderolide (11), and (-) sclerodione (12).. The structures of these closely related compounds were established from extensive spectroscopic investigations on the basis of one and two dimensional NMR spectroscopic studies (H, C, COSY, NOESY, HSQC and HMBC) as well as mass spectrometric analysis (LC/MS, HREIMS and HRESIMS), UV and IR spectra.. Compounds 5 and 11 showed the same antimicrobial activity toward Staphylococcus aureus SG 511 with an MIC value of 24 mM.

    Article Title: Doubly prenylated tryptamines: cytotoxicity, antimicrobial activity and cyclisation to the marine natural product flustramine A.
    Article Snippet: The marine natural product flustramine A was synthesised via oxidative cyclisation of Nb-methylated 1-prenyl-2-tert-prenyl-6-bromotryptamine and subsequent reduction of the resulting amidinium salt.. Only the tert-prenyl group migrated, whereas the 1-prenyl group remained in place.. Interestingly, the 2-tertprenylated precursor revealed to be the biologically most active of our entire series of 21 compounds.

    Article Title: Fluorescent analogs of the marine natural product psammaplin A: synthesis and biological activity.
    Article Snippet: The symmetrical disulfide psammaplin A from the marine sponge Pseudoceratina sp. was synthesized and structurally altered by replacement of one of the α-(hydroxyimino)acyl units by a fluorescent 4-coumarinacetyl moiety.. Thus, the first fluorescent analogs of psammaplin Awere obtained.. Structural variation also covered the substitution pattern of the phenyl ring.

    Article Title: Synthesis and Cytotoxicity of a Diazirine-Based Photopsammaplin
    Article Snippet: The first photoreactive derivative of the cytotoxic marine natural product psammaplin A (1) has been synthesized by appending 1-azi-2,2,2-trifluoroethyl moieties at both benzene rings.. Photopsammaplin 8 exhibited anticancer activity in vitro with a geomean IC50 value of 1.4 μM in a panel of 42 human cancer cell lines.. Particularly sensitive cell lines were found among bladder, lung, mammary, and prostate cancer as well as melanoma (MEXF 276), with IC50 values 0.6 μM.

    In Vitro:

    Article Title: The Role of Formamidine Groups in Dextran Based Nonviral Vectors for Gene Delivery on Their Physicochemical and Biological Characteristics.
    Article Snippet: .. In Vitro Cytotoxicity Test: In vitro cytotoxicity of the polymers was tested by the MTT assay[56,48] using L-929 mouse fibroblasts (ACC 2, DSMZ) cultured in RPMI (Roswell Park Memorial Institute) 1 640 culture medium (Gibco, Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal bovine serum at 37 °C, 5% CO2 and 95% relative humidity. .. Briefly, after 24 h preincubation cells (8500 per well, Cellstar 96-well plates, Greiner Bio-One GmbH, Frickenhausen, Germany) were incubated with serial dilutions of the polymers (3.91 μg mL−1 to 500 μg mL−1) for 4 h. After removal of the supernatant and replacement by 200 μL serum containing RPMI 1640 medium cells were incubated for further 20 h. Following the incubation with MTT solution (500 μg mL−1), the absorbance of the samples was measured in a microplate reader (Spark 10M multimode microplate reader, Tecan Group AG) at 570 nm.

    Cell Culture:

    Article Title: The Role of Formamidine Groups in Dextran Based Nonviral Vectors for Gene Delivery on Their Physicochemical and Biological Characteristics.
    Article Snippet: .. In Vitro Cytotoxicity Test: In vitro cytotoxicity of the polymers was tested by the MTT assay[56,48] using L-929 mouse fibroblasts (ACC 2, DSMZ) cultured in RPMI (Roswell Park Memorial Institute) 1 640 culture medium (Gibco, Thermo Fisher Scientific, Waltham, USA) supplemented with 10% fetal bovine serum at 37 °C, 5% CO2 and 95% relative humidity. .. Briefly, after 24 h preincubation cells (8500 per well, Cellstar 96-well plates, Greiner Bio-One GmbH, Frickenhausen, Germany) were incubated with serial dilutions of the polymers (3.91 μg mL−1 to 500 μg mL−1) for 4 h. After removal of the supernatant and replacement by 200 μL serum containing RPMI 1640 medium cells were incubated for further 20 h. Following the incubation with MTT solution (500 μg mL−1), the absorbance of the samples was measured in a microplate reader (Spark 10M multimode microplate reader, Tecan Group AG) at 570 nm.



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